![]() ![]() Thus, with these material modifications, the reliability and efficacy of the current RapidHIT ID System need to be evaluated. These modifications included configuration of the primary cartridge and other hardware, such as the heated optics and thermal cycling (personal communication), modifications to thermal cycling parameters and available software tools (personal communication), introduction of an additional (RapidINTEL) sample cartridge, and changes to the thresholds used when making genotype calls. In the past few years, the RapidHIT ID instrument has been modified again compared with the previous versions. Previous changes/updates to the RapidHIT ID System instrumentation are the use of different PCR STR typing kits and a single sample analysis design. The other is the Applied Biosystems RapidHIT ID System (Thermo Fisher Scientific, Waltham, MA, USA), which was formerly a product of IntegenX (Pleasanton, CA, USA). One is the ANDE Rapid DNA Identification System (ANDE Corporation, Longmont, CO, USA), which was formerly known as the NetBio DNAScan Rapid DNA Analysis System. Two primary Rapid DNA analysis platforms currently exist and are being evaluated and employed by forensic laboratories and police agencies. Rapid DNA Systems collectively include a Rapid DNA instrument, the PCR STR typing kit/Rapid DNA cartridge, and an expert system software all used to develop and interpret an STR profile. Because of the greater reliance on the system in such settings, data are needed to define the performance and limitations of commercially available Rapid DNA systems. ![]() However, operation of Rapid DNA Systems in non-traditional laboratory settings relies on operators with widely varying backgrounds and not necessarily having the training and education of scientists. The benefit of Rapid DNA Systems, with placement in non-traditional laboratory settings, is the potential to decrease the turn-around time from sample collection through sample processing. Rapid DNA Systems can be placed in both laboratory and non-laboratory environments that each play a role in investigations, prosecutions, and the subsequent care of survivors, such as booking stations, customs and border patrol stations, the site of mass disasters, or hospitals for tracking biopsy samples. Rapid DNA Systems offer the forensic community a fully automated system capable of processing DNA from a biological sample and interpreting the results in approximately 90 min with minimal human intervention. However, forensic scientists have continually worked towards reducing the amount of time and labor needed to generate these STR profiles (e.g., updates and developments in automation, direct amplification, polymerase chain reaction (PCR) protocols, etc.) as the demand on forensic resources, including trained laboratory personnel, continues to increase while reduced turnaround times are sought. Forensic laboratories currently use capillary electrophoresis (CE)-based technologies and workflows to generate STR profiles from biological evidence. Short tandem repeat (STR) typing is routinely used to associate or exclude individuals as possible contributors of biological evidence. Overall, the current Applied Biosystems RapidHIT ID Instrument with the ACE GlobalFiler Express sample cartridge was found to be a reliable tool for generation of STR profiles from reference-type buccal swabs. These validation studies included assessments of sensitivity, contamination, concordance, reproducibility and repeatability, stability, inhibition, mixtures, sample reprocessing, precision, and first-pass success rate. ![]() Thus, validation studies of a Rapid DNA workflow consisting of the Applied Biosystems RapidHIT ID Instrument and RapidLINK software with a focus on the ACE GlobalFiler Express Sample Cartridge and reference buccal swabs were performed in accordance with Scientific Working Group on DNA Analysis Methods Validation Guidelines. With a greater reliance on the system than on the analyst, validation data are especially needed to define the performance and limitations of commercially available Rapid DNA systems. Rapid DNA platforms are fully automated systems capable of processing DNA from biological samples and interpreting the results in approximately 90 minutes with minimal human intervention. ![]()
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